Roche Transcriptor First Strand cDNA Synthesis Kit, 50 reactions
The Transcriptor First Strand cDNA Synthesis Kit provides all reagents required for first-strand cDNA synthesis reactions up to 14kb on all real-time instruments and conventional thermal cyclers. Three different priming methods can be used. The enclosed anchored-oligo(dT)18 primer is designed to bind at the beginning of the poly(A) tail and guarantees full-length cDNA synthesis. Transcriptor Reverse Transcriptase - the core component of the kit - has RNA-directed DNA polymerase activity, DNA-dependent DNA polymerase activity, unwinding activity, and RNase H activity that digests RNA in RNA:DNA hybrids. The latter circumvents the need to perform an additional time-consuming RNase H incubation step after reverse transcription. This shortens the reaction time and reduces costs. Thermostable Protector RNase Inhibitor is included in the kit to protect RNA from degradation at high reaction temperatures
Applications
The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications:
- Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments
- Generating cDNA libraries with large and full-length inserts
- Cloning genes of interest
Contents
50 reactions, including 10 control reactions
- Transcriptor Reverse Transcriptase: 1 vial, 25 μl
- Transcriptor RT Reaction Buffer 5x conc: 1 vial. 1 ml
- Protector RNase Inhibitor: 1 vial , 50 μl (40 U/μl)
- Deoxynucleotide Mix: 1 vial, 100 μl
- Anchored-oligo(dT)18 Primer: 1 vial, 100 μl (50 μM)
- Random Hexamer Primer: 1 vial, 100 μl (600 μM)
- Control RNA: 1 vial , 20 μl (50ng/μl)
- Control Primer Mix PBGD: 1 vial, 40 μl, (5 μM)
- Water, PCR Grade: 1 vial, 1ml
